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| 产品名称： | lambdaTRP |
|---|---|
| 商品货号： | TS145327 |
| Designations： | lambdaTRP |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 42.40 Vector: lambdaTRP (phage, lambda - replacement) Promoters: Promoter for expression GAL1 Construction: lambdaYES-R, pTRP Marker(s):TRP1,ampR Construct size (kb): 42.40 Features: marker(s): TRP1 marker(s): ampR other: left arm (19.6 kb) other: lox other: right arm (15.2 kb, with cI857) promoter for expression: GAL1 replicon: 2 micron replicon: oriT replicon: pMB1 MCS: EcoRI...SacI transcription terminator: CYC1 |
| Applications： | expression vector lambda vector allowing conversion to plasmid vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--30.0, 18.0; SalI--20.0, 14.0, 13.0. Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the CYC1 terminators. The GAL1 promoter lacks a translational start signal. There is also some expression on glucose. Vector for the construction of cDNA libraries permitting regulated expression in yeast and simple conversion to plasmid via the cre-lox system. The plasmid derivative (pTRP, 8.0 kb) is a YE-type shuttle expression vector. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081 Stephen J Elledge, personal communication |
| Shipped： | freeze-dried |