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| 产品名称： | pJAK12 JK386 |
|---|---|
| 商品货号： | TS145373 |
| Designations： | pJAK12 JK386 |
| Depositors： | JA Kornacki |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli SCS1 |
| Vector Information： | Size (kb): 10.8000001907348600 DESCRIPTION OF VECTOR: Intact vector size: 10.800 Type of vector: plasmid Cloning sites: EcoRI SacI Ecl136II KpnI SmaI XmaI BamHI XbaI SalI PstI HindIII Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI HincII PstI SphI HindIII Construction: pMMB67EH (ATCC 37622), R100.1 Host range: broad host range; Escherichia coli Features (with orientation and position when available): promoter: tac, -> MCS: EcoRI...HindIII, -> transcription terminator: rrnB restriction site: SphI marker(s): strR (spcR) replicon: oriV replicon: oriT replicon: RSF1010 repressor gene: lacIq Vector: pJAK12 (plasmid) Promoters: Promoter tac Construction: pMMB67EH (ATCC 37622), R100.1 Marker(s):strR (spcR) Construct size (kb): 10.80000019073486 Features: marker(s): strR (spcR) promoter: tac replicon: RSF1010 replicon: oriT replicon: oriV repressor gene: lacIq MCS: EcoRI...HindIII restriction site: SphI transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--10.8; HindIII--10.8; SphI--9.7, 1.1. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. |
| Media： | ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication |
| Related Products： | component of:ATCC 87055 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |