| 产品名称: | HFBCC69 |
|---|---|
| 商品货号: | TS145377 |
| Designations: | HFBCC69 |
| GenBank Number: | M78471 |
| Species: | Homo sapiens, human |
| Depositors: | JC Venter |
| Vector: | Construct size (kb): 4.650000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: USF1 Genomic copy number: unique Gene name: upstream transcription factor 1 Contains complete coding sequence?: U Chromosome: 1; Localization: 1 q22-q23 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.7 Cross references: DNA Seq. Acc.: M78471; EST: EST00619; Primer: M13 Forward Insert lengths(kb): 1.700000047683716 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: upstream transcription factor 1(transcription factor USF) USF1 |
| Insert Size (kb): | 1.700 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.7. Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.7. According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00619 may contain coding sequence: 109-68 (good). EST00619 matches GenBank accession X55666 with 97% identity over a length of 276 nt. |
| References: | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 J C Venter, personal communication |