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| 产品名称： | pIC-ER-C pIC 20 ER N11 |
|---|---|
| 商品货号： | TS145384 |
| Designations： | pIC-ER-C pIC 20 ER N11 |
| GenBank Number： | M12674 |
| Species： | Homo sapiens, human |
| Depositors： | H Ahrens |
| Applications： | produces protein estrogen receptor |
| Vector： | Construct size (kb): 3.759999990463257 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pIC20H Intact vector size: 2.722 Type of vector: plasmid Vector end: SmaI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV BglII XhoI NruI Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L08912 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ESR Genomic copy number: unique Gene name: estrogen receptor Contains complete coding sequence?: U Chromosome: 6; Localization: 6 q25.1 Type of DNA: cDNA Insert 5 end: NaeI Insert 3 end: EcoRI Insert size (kb): 1.06 Cross references: DNA Seq. Acc.: M12674 Insert lengths(kb): 1.059999942779541 Gene product: estrogen receptor ESR Alleles: A1, A2 Target Gene: estrogen receptor |
| Insert Size (kb)： | 1.060 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9; BglII--3.3, 0.54; HindIII--2.6, 0.88, 0.33; PstI--3.2, 0.64; XbaI--3.4, 0.43; HindIII/SstI--2.7, 0.76, 0.34. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.55; HindIII--0.30; NcoI--0.46; PstI--0.60, 0.77; StuI--0.76; XbaI--0.41. Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies. It binds estrogens and antiestrogens. Contains coding sequence for amino acids 241-595 including the stop codon. The recombinant fusion protein contains an additional 18 N-terminal amino acids encoded by the vector polylinker. |
| References： | Ahrens H, et al. Overproduction of full-length and truncated human estrogen receptors in Escherichia coli. Receptor 2: 77-92, 1992. PubMed: 1472947 |