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微信小章| 产品名称： | Aspergillus parasiticus Speare |
|---|---|
| 商品货号： | TS145393 |
| Strain Designations： | Su-1 NRRL 5862 |
| Application： | Produces aflatoxins |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | Highly aflatoxigenic
Genome sequencing strain (J. Craig Venter Institute, USA) |
| Medium： | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 312: Czapeks agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence CATTACCGAGTGTAGGGTTCCTAGCGAGCCCAACCTCCCACCCGTGTTTACTGTACCTTAGTTGCTTCGGCGGGCCCGCCGTCATGGCCGCCGGGGGCGTCAGCCCCGGGCCCGCGCCCGCCGGAGACACCACGAACTCTGTCTGATCTAGTGAAGTCTGAGTTGATTGTATCGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAGTGTGAATTGCAGAATTCCGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCATCAAGCACGGCTTGTGTGTTGGGTCGTCGTCCCCTCTCCGGGGGGGACGGGCCCCAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCTTGCCGAACGCAAAACAACCATTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG D1D2 region of the 28/26S ribosomal RNA gene AATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGCAGAGGATGCTTCGGGTGCGGCCCCTGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCTCCAGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCTGGGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCTCCCGGAATGTAGTGCCCTCCGGGGCACCTTATAGCCGGGAGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGA |
| Name of Depositor： | JW Bennett |
| Cross References： | Nucleotide (GenBank) : Z47198 A.parasiticus pksA gene for polyketide synthase. Nucleotide (GenBank) : L22177 Aspergillus parasiticus zinc finger (apa-2) gene. Nucleotide (GenBank) : L26220 Aspergillus parasticus (aflR) gene, complete cds. Nucleotide (GenBank) : AF002660 Aspergillus parasiticus AFLJ (aflJ) gene, complete cds. Nucleotide (GenBank) : U51328 Aspergillus parasiticus versicolorin B synthase (vbs) mRNA, Nucleotide (GenBank) : U38948 Aspergillus parasiticus nitrate reductase (niaD) gene, complete Nucleotide (GenBank) : L26222 Aspergillus parasiticus regulatory protein (af1R) mRNA, complete Nucleotide (GenBank) : AF268071 Aspergillus parasiticus aflatoxin efflux pump AFLT (aflT) gene, Nucleotide (GenBank) : AF148539 Aspergillus parasiticus major nitrogen regulatory protein (areA) Nucleotide (GenBank) : U39557 Aspergillus parasiticus nitrite reductase (niiA) gene, partial cds. |
| References： | Bennett JW, et al. Identification of averantin as an aflatoxin B1 precursor: placement in the biosynthetic pathway. Appl. Environ. Microbiol. 39: 835-839, 1980. PubMed: 7377778 Mahanti N, et al. Structure and function of fas-1A, a gene encoding a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl. Environ. Microbiol. 62: 191-195, 1996. PubMed: 8572694 McGuire SM, et al. Purification and characterization of versicolorin B synthase from Aspergillus parasiticus. Catalysis of the stereodifferentiating cyclization in aflatoxin biosynthesis essential to DNA interaction. Biochemistry 35: 11470-11486, 1996. PubMed: 8784203 Silva JC, et al. Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. J. Biol. Chem. 271: 13600-13608, 1996. PubMed: 8662689 Mayne RY, et al. Instability of an aflatoxin-producing strain of Aspergillus parasiticus. Mycologia 63: 644-648, 1971. PubMed: 5563952 Yu J, Bhatnagar D, Cleveland TE. Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus. FEBS Lett. 564: 126-130, 2004. PubMed: 15094053 Yu J, et al. Clustered pathway genes in aflatoxin biosynthesis. Appl. Environ. Microbiol. 70: 1253-1262, 2004. PubMed: 15006741 Chang PK, Yu J. Characterization of a partial duplication of the aflatoxin gene cluster in Aspergillus parasiticus TS145393. Appl. Microbiol. Biotechnol. 58: 632-636, 2002. PubMed: 11956746 Yu J, et al. Cloning and functional expression of an esterase gene in Aspergillus parasiticus. Mycopathologia 156: 227-234, 2002. PubMed: 12749588 Yu J, et al. Genes encoding cytochrome P450 and monooxygenase enzymes define one end of the aflatoxin pathway gene cluster in Aspergillus parasiticus. Appl. Microbiol. Biotechnol. 53: 583-590, 2000. PubMed: 10855719 Chang PK, et al. adhA in Aspergillus parasiticus is involved in conversion of 5-hydroxyaverantin to averufin. Appl. Environ. Microbiol. 66: 4715-4719, 2000. PubMed: 11055914 Yu J, et al. Cloning of a sugar utilization gene cluster in Aspergillus parasiticus. Biochim. Biophys. Acta 1493: 211-214, 2000. PubMed: 10978525 Yu J, et al. Cloning and characterization of avfA and omtB genes involved in aflatoxin biosynthesis in three Aspergillus species. Gene 248: 157-167, 2000. PubMed: 10806361 Chang P-K, et al. Characterization of Aspergillus parasiticus major nitrogen regulatory gene, areA. Biochim. Biophys. Acta 1491: 263-266, 2000. PubMed: 10760588 |