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微信小章| 产品名称： | 18-26-4 |
|---|---|
| 商品货号： | TS145467 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | B lymphocyte; hybridoma |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dengue |
| Applications： | The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| Clinical Data： | Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on 3H5-1 (monoclonal antibody against Dengue virus-2) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on 3H5-1 (monoclonal antibody against Dengue virus-2) |
| Comments： | Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgM |
| Name of Depositor： | D.G. Sieckmann |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |