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| 产品名称： | OMRF2189 OMRF 2189 |
|---|---|
| 商品货号： | TS145483 |
| Designations： | OMRF2189 OMRF 2189 |
| Species： | Mus musculus, mouse |
| Depositors： | JM Gimble |
| Vector： | Construct size (kb): 8.149998664855957 |
| Insert： | DNA: genomic Insert lengths(kb): 5.199999809265137 Tissue: liver Gene product: interleukin 6 (interferon, beta 2)(interleukin 6) Il6 |
| Insert Size (kb)： | 5.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SstI--5.2, 3.0; EcorI--4.4, 3.8; HindIII--6.6, 1.6; BamHI--8.2; XbaI--6.0, 2.2. The insert includes the following restriction sites (in approximate kb from the 5 end): EcoRI--0.2; HincII--2.3; XbaI--3.0; BglII--3.3. The insert includes exons 1, 2, 3 and upstream and downstream sequences. Exons 1 and 2 can be excised by a HincII/XbaI double digest yielding a 750 bp fragment. The genomic copy number for the entire insert is not unique. There is a repetitive element located within the third intron. The HincII/XbaI fragment does detect a unique DNA fragment. Transcription from the T3 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Jeffrey M Gimble, personal communication |