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微信小章| 产品名称： | phAT4.8 phAT4.4 |
|---|---|
| 商品货号： | TS145488 |
| Designations： | phAT4.8 phAT4.4 |
| GenBank Number： | K02212 |
| Species： | Homo sapiens, human |
| Depositors： | SL Woo |
| Vector： | Construct size (kb): 11.80000019073486 |
| Insert： | DNA: genomic Insert lengths(kb): 4.800000190734863 Tissue: liver Gene product: alpha-1-antitrypsin (protease inhibitor) PI Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1 |
| Insert Size (kb)： | 4.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 4.8; BamHI--10.6, 1.2; HindIII--4.2, 3.6, 3.5, 0.7; PvuII--6.5, 2.7, 1.75, 0.85; PstI--3.4, 3.4, 1.3, 0.8, 0.5. The insert contains 2 BamHI sites (upstream of exon 1) and 2 HindIII sites (in intron 1). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert is the genomic EcoRI fragment containing 5 flanking sequences, exon 1, and part of intron 1. |
| References： | Long GL, et al. Complete sequence of the cDNA for human alpha1-antitrypsin and the gene for the S variant. Biochemistry 23: 4828-4837, 1984. PubMed: 6093867 Sifers RN, et al. A frameshift mutation results in a truncated alpha1-antitrypsin that is retained within the rough endoplasmic reticulum. J. Biol. Chem. 263: 7330-7335, 1988. PubMed: 3259232 Cox DW, et al. DNA restriction fragments associated with alpha1-antitrypsin indicate a single origin for deficiency allele PI Z. Nature 316: 79-81, 1985. PubMed: 2989709 |