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| 产品名称： | phHK15-2 |
|---|---|
| 商品货号： | TS145531 |
| Designations： | phHK15-2 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 6.46999979019165 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.200000047683716 Tissue: kidney Gene product: hexokinase 1 HK1 Alleles: A2, A1 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.3 (doublet); EcoRI/PstI--3.3, 2.7, 0.8; HindIII--4.2, 2.5; BamHI--3.4, 2.0, 1.0; PstI--6.6. This clone encodes amino acids 92-917 and includes 765 bp of 3-untranslated sequence. The insert was derived from a 3.25 kb EcoRI fragment of lambdahHEX-15 (ATCC 61510). Enzyme(s) not detecting polymorphism: BclI, BglI, BglII, EcoRI, MspI, PstI, RsaI, SphI, TaqI, XbaI in a panel of 14 unrelated individuals. |
| References： | Nishi S, et al. HincII RFLP at the human hexokinase I (HK1) locus on chromosome 10. Nucleic Acids Res. 17: 7547, 1989. PubMed: 2571978 Nishi S, et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157: 937-943, 1988. PubMed: 3207429 Graeme I Bell, personal communication |