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| 产品名称： | pKL19-2 |
|---|---|
| 商品货号： | TS145573 |
| Designations： | pKL19-2 |
| Depositors： | New England Biolabs, Inc., KD Lunnen, New England Biolabs, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.6900000572204590 Vector: pKL19-2 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):ampR Construct size (kb): 2.690000057220459 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none enhancer: none |
| Applications： | expression vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7. Expression vector permitting production of a fusion protein and visual detection of recombinants, using the beta-galactosidase alpha peptide as the reporter group. Distributed in aliquots of 2 ug (100 ng/ul). A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated dpCCCCGGGG. |
| References： | Lunnen KD, Wilson GG. Method for producing the XmaI restriction endonuclease and methylase. US Patent 5,002,882 dated Mar 26 1991 Keith D Lunnen, personal communication |
| Shipped： | freeze-dried |