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| 产品名称： | Human rhinovirus 9 |
|---|---|
| 商品货号： | TS145594 |
| Classification： | Picornaviridae, Rhinovirus |
| Agent： | Human rhinovirus 9 |
| Strain： | 211 211-CV 13 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This product is a mycoplasma free preparation derived from ATCC VR-489. |
| Product Format： | frozen |
| Storage Conditions： | Store at xa0-70°C or colder |
| Comments： | This product is a mycoplasma free preparation derived from ATCC VR-489. |
| Effect on Host： | Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host:H1-HeLa (ATCC CRL-1958) Recommended Host: xa0H1HeLa (ATCC® CRL-1958x99) Alternate Hosts:HeLa (ATCC CCL-2), WI-38 (ATCC CCL-75), MRC-5 (ATCC CCL-171) |
| Growth Conditions： | Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1. Adsorb 1 hour at 33.0°C in a humidified, 5% CO2 atmosphere with continuous rocking. End adsorption by adding virus growth medium. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 3-7 days with rocking, until CPE are well advanced in 100% of the culture. |
| Name of Depositor： | VV Hamparian, RM Conant |
| Source： | Presumed from human throat washings; derived from ATCC VR-489 following treatment to remove mycoplasma contamination. |