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微信小章| 产品名称： | pYNZ22 pYNZ22.1 |
|---|---|
| 商品货号： | TS145768 |
| Designations： | pYNZ22 pYNZ22.1 |
| Species： | Homo sapiens, human |
| Applications： | RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 5.949999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.700000047683716 Gene product: DNA Segment, single copy D17S5 |
| Insert Size (kb)： | 1.700 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--5.95; PstI--5.95; HindIII--5.95; EcoRI--5.95; BamHI--4.4, 1.6. pYNZ22 (ATCC 57574) and pYNH37 (ATCC 57568) map to the same 30 kb SfiI fragment. This clone is of the same DNA segment as pJCZ16.2 (ATCC 57658) and detects the same polymorphism. The MspI polymorphism demonstrates codominant segregation in more than 60 families. RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: BglII, EcoRI. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pYNZ22) on chromosome 17p D17S30. Nucleic Acids Res. 16: 5707, 1988. PubMed: 2455284 vanTuinen P, et al. Molecular detection of microscopic and submicroscopic deletions associated with Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 587-596, 1988. PubMed: 3189330 Schwartz CE, et al. Detection of submicroscopic deletions in band 17p13 in patients with the Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 597-604, 1988. PubMed: 2903661 Horn GT, et al. Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Res. 17: 2140, 1989. PubMed: 2928126 Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351 Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152 Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130 Yusuke Nakamura, personal communication |