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| 产品名称： | K3-1.1 |
|---|---|
| 商品货号： | TS145797 |
| Designations： | K3-1.1 |
| Species： | Mus musculus, mouse |
| Depositors： | F Takei, KJ Horley |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.029999971389771 Tissue: myeloma NS-1 cell line Gene product: intercellular adhesion molecule 1 Ly47 |
| Insert Size (kb)： | 3.030 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites separated by (bp): EcoRI- 548- Asp700I- 950- PvuII- 147- HindIII- 547- PvuII- 640- PvuII- 145- Asp700I- 54- EcoRI. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--3.05, 2.95; HindIII--4.3, 1.65; PstI--3.3, 1.85, 0.6; SmaI--5.8. Clones K4-1.1 (ATCC 63043) and K3-1.1 (TS145797) differ in the 5 end of the insert. Nt 638 of the K3-1.1 insert equals nt 132 of the K4-1.1 insert. The insert has an open reading frame of 1593 nt, 552 nt of 5 untranslated sequence and 851 nt of 3 untranslated sequence. It has an atypical N-terminal sequence and may represent an alternative splicing product. The overall protein structure is quite similar to human ICAM1 and there is 50% homology at the amino acid level. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Horley KJ, et al. Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 8: 2889-2896, 1989. PubMed: 2573511 Fumio Takei, personal communication |