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| 产品名称： | p3.4 p3.4BHI |
|---|---|
| 商品货号： | TS145889 |
| Designations： | p3.4 p3.4BHI |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IGHJ Gene name: immunoglobulin heavy polypeptide, joining region Contains complete coding sequence?: U Chromosome: 14; Localization: 14 q32.33 Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 3.4 Cross references: Insert lengths(kb): 3.400000095367432 Gene product: immunoglobulin heavy polypeptide, joining region IGHJ Alleles: A1, A2, A3, A4 |
| Insert Size (kb)： | 3.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.4; BglII--6.4, 1.4; EcoRI--7.8; EcoRV--7.8; PstI--7.8. This fragment originates 1.25 kb 5 to a BglII site just 5 to the J(H) sequences. Subcloned from Ch28-6. Enzymes(s) not detecting polymorphism: SstI, EcoRV, EcoRI, HaeIII, PvuII, PstI, TaqI, NdeII. |
| References： | Silva AJ, et al. Characterization of a highly polymorphic region 5 to JH in the human immunoglobulin heavy chain. Nucleic Acids Res. 15: 3845-3857, 1987. PubMed: 2884636 Raymond L White, personal communication |