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| 产品名称： | pCSF25/5 |
|---|---|
| 商品货号： | TS145926 |
| Designations： | pCSF25/5 |
| GenBank Number： | M20021 |
| Species： | chloris striate mosaic geminivirus (CSMV) |
| Depositors： | BA Morris |
| Vector： | Construct size (kb): 5.40 |
| Insert： | DNA: Genomic
Insert lengths (kb): 2.75
Tissue: DNA from infected grass (Dactyloctenium australe) |
| Insert Size (kb)： | 2.75 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 2.7; KpnI--4.9, 0.5; PstI--3.15, 1.4, 0.87; SacI--5.4.
The insert contains the following restriction sites (approximate kb from a BamHI site): EcoRI--2.17; KpnI--0.23, 0.69; PstI--0.42, 1.32; SacI--0.94.
Features of the virus occupy the following positions: LIR--(2-233); SIR--(1376-1579); ORF 33.1--(2750-1867); ORF 15.8--(1987-1580); ORF 26.8--(658-1335); ORF 14.5--(234-1375).
Constructed by linearizing the viral ds DNA at the unique BamHI site (nt 351) within the ORF encoding a 14.5 kDa protein. |
| Classification： | Geminivirus |
| References： | Andersen MT, et al. Nucleotide sequence of the Geminivirus chloris striate mosaic virus. Virology 164: 443-449, 1988. PubMed: 3369088 Morris BA, et al. Plant Mol. Biol. 11: 759-803, 1988. Bret A Morris, personal communication |