微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBS-Cip1 |
|---|---|
| 商品货号： | TS145950 |
| Designations： | pBS-Cip1 |
| GenBank Number： | L25610 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Vector： | Construct size (kb): 5.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript Intact vector size: 2.960 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: Polylinker sites: Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDKN1A Other gene symbols: CIP1; WAF1* Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1) Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 p21.2 Tissue: B lymphocyte (EBV-transformed) Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 2.1 Cross references: DNA Seq. Acc.: L25610 Insert lengths(kb): 2.099999904632568 Tissue: B lymphocyte (EBV transformed) Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) CIP1 |
| Insert Size (kb)： | 2.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.2, 0.66, 0.58, 0.50, 0.34, 0.22; EcoRI--3.8, 1.2; KpnI--3.8, 0.92, 0.31; PvuII--2.8, 1.2, 0.92, 0.4; XhoI--3.0, 2.1. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.14, 0.61, 1.25, 1.8; KpnI--0.92; EcoRI--1.0; XhoI--2.0. Contains the complete coding sequence (nt 79-573 of the sequence record). |
| References： | Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 |