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| 产品名称： | pDSV100 |
|---|---|
| 商品货号： | TS146008 |
| Designations： | pDSV100 |
| GenBank Number： | M23022 |
| Species： | Digitaria streak virus |
| Depositors： | PM Mullineaux, GP Accotto |
| Vector： | Construct size (kb): 3.98799991607666 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8799999952316284 Gene product: coat protein V2 |
| Insert Size (kb)： | 0.880 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--4.0; HindIII--4.0; SalI--4.0; PstI--3.3, 0.7. The insert contains the following restriction sites (nt position according the published sequence): KpnI--249, 674, 1008; PstI--454; SacI--506; NruI--883. Linearizing with SphI or HindIII and transcription with SP6 RNA polymerase will give the sense strand. The SP6-derived transcript would direct the translation of the V2 (coat protein) ORF in an in vitro translation system, such as wheat germ or rabbit reticulocyte lysate. Constructed from the MluI/SpeI fragment extending from nt 183 to 1071 of the published sequence. The EcoRI/MluI and SpeI/XbaI sites were destroyed. |
| Classification： | Geminivirus |
| References： | Donson J, et al. The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology 161: 160-169, 1987. PubMed: 3672928 P M Mullineaux, personal communication |