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| 产品名称： | c9866 9866 |
|---|---|
| 商品货号： | TS146072 |
| Designations： | c9866 9866 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Applications： | The ATCC encourages users of this material to communicate their results to us. |
| Vector： | Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene name: DNA Segment Contains complete coding sequence?: U Chromosome: II; Localization: II L Strain: AB972 (ATCC 76269) Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): Cross references: Gene product: DNA Segment |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | The insert contains EcoRI/HindIII fragments of the following sizes (kb): 0.406, 0.445, 0.533, 0.536, 0.590, 0.614, 0.653, 0.656, 0.659, 0.701, 0.702, 0.936, 0.990, 1.180, 1.193, 1.317, 1.321, 1.424, 1.469, 2.026, 2.155, 2.232, 2.513, 2.568, 2.658, 2.955. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70066, 70272, 70549, 70960, 71067, 71107, 71126, 71150. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |