微信小陈
微信小章| 产品名称： | P-9 |
|---|---|
| 商品货号： | TS146092 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain. The antibody reacts with the large subunit of human mu-calpain. . It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with mu-calpain isolated from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the large subunit of human mu-calpain.. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain.
The antibody does not cross-react with human placental m-calpain PubMed: 8702541. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain. |
| Comments： | Animals were immunized with mu-calpain isolated from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the large subunit of human mu-calpain.. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain.
The antibody does not cross-react with human placental m-calpain PubMed: 8702541. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.xa0 Attached cells may be subcultured by scraping. Medium Renewal:xa0 Add fresh medium every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | kappa |
| Name of Depositor： | R Lane, RL Mellgren |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1990 |
| References： | Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |