微信小陈
微信小章| 产品名称： | pDM-17 pDM17 |
|---|---|
| 商品货号： | TS146146 |
| Designations： | pDM-17 pDM17 |
| Species： | Homo sapiens, human |
| Depositors： | M Barbacid, D Martin-Zanco |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.210000038146973 Tissue: colon carcinoma Gene product: neurotrophic tyrosine kinase, receptor, type 1( Oncogene TRK, neutrotrophic tyrosine receptor kinase 1 (trk proto-oncogene), proto-oncogene trk) NTRK1 |
| Insert Size (kb)： | 1.210 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; EcoRI/BamHI--1.1, 2.7. pDM10-1 was isolated from a cDNA library from a third cycle NIH/3T3 transformant derived from a human colon carcinoma. The insert includes nucleotides +854 to +2064. This region includes tyrosine kinase sequences, but not tropomyosin sequences. This was constructed by inserting the BalI-EcoRI fragment of pDM10-1 into the SmaI-EcoRI sites of the pUC18 polylinker. The insert can be isolated by a BamHI/EcoRI double digest. |
| References： | Martin-Zanca D, et al. A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences. Nature 319: 743-748, 1986. PubMed: 2869410 Coulier F, et al. Human trk oncogenes activated by point mutation, in-frame deletion, and duplication of the tyrosine kinase domain. Mol. Cell. Biol. 10: 4202-4210, 1990. PubMed: 1695324 Klein R, et al. The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 65: 189-197, 1991. PubMed: 1849459 Mariano Barbacid, personal communication |