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微信小章| 产品名称： | Tf TF66G2 |
|---|---|
| 商品货号： | TS146221 |
| Designations： | Tf TF66G2 |
| GenBank Number： | M12525 |
| Species： | Homo sapiens, human |
| Depositors： | G Lucotte |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.0 Gene product: transferrin TF Alleles: B1, B2, A1, A2 |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.3, 0.8, 0.3; EcoRI--uncut; PvuII--5.6; BamHI--5.6; EcoRI/PvuII--5.6. The insert contains an internal PstI site (two fragments of 787 and 214 bp). The insert encodes TF from amino acid 403 to COOH terminus with 166 nucleotides of 3 untranslated region. Incomplete dominance for the PstI RFLP has been demonstrated in one informative family. HindIII variant shows amplification of TF sequences. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Enzyme(s) not detecting polymorphism: EcoRI, PvuII, BamHI, TaqI, MspI. |
| References： | Uzan G, et al. Molecular cloning and sequence analysis of cDNA for human transferrin. Biochem. Biophys. Res. Commun. 119: 273-281, 1984. PubMed: 6322780 le Paslier D, et al. Evolution comparee du gene transferrine chez lhomme et les primates, par hybridation apres transfert avec une sonde specifique. Biochem. Syst. Ecol. 13: 207-212, 1985. Lavareda de Souza S, Lucotte G. Genetique moleculaire de la transferrine: Localisation chromosomique et variation individuelle du gene. Bio-sciences 4: 139-144, 1985. Barbara Weiffenbach, personal communication Gerard Lucotte, personal communication |