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| 产品名称： | pPI-MG2 |
|---|---|
| 商品货号： | TS146295 |
| Designations： | pPI-MG2 |
| Species： | Homo sapiens, human |
| Depositors： | NIH, M Brantly, NIH |
| Applications： | in another host, produces protein alpha-1-antitrypsin (protease inhibitor) |
| Vector： | Construct size (kb): 11.4379997253418 |
| Insert： | DNA: genomic Insert lengths(kb): 2.700000047683716 Gene product: alpha-1-antitrypsin (protease inhibitor) PI Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1 Target Gene: alpha-1-antitrypsin (protease inhibitor) |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.75 (doublet), 0.70; EcoRV--11.5; HindIII--5.8, 4.5, 1.05. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--2.26. A minigene constructed from genomic sequence beginning 50 bp upstream of the start ATG (exon 2) and extending into exon 3, ligated to a cDNA fragment encoding exons 3-5. |
| References： | Laubach VE, et al. Characterization of a human alpha-1-antitrypsin null allele involving aberrant mRNA splicing. Hum. Mol. Genet. 2: 1001-1005, 1993. PubMed: 8364536 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |