Construct size (kb): 5.800000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TEGT
Genomic copy number: unique
Gene name: testis enhanced gene transcript
Contains complete coding sequence?: N
Chromosome: 12
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: T08698; EST: EST06590; Primer: M13-21; THC
id: THC123576
DNA Seq. Acc.: T08699; EST: EST06591; Primer: M13 Reverse;
THC id: THC123576
DNA Seq. Acc.: X75861; EST: EST06591
Nucleotides 1-363 of the insert correspond to
nucleotides 58-420 of X75861.
DNA Seq. Acc.: X75861; EST: EST06590
Nucleotides 2174-2509 of the insert correspond to
nucleotides 2231-2566 of X75861 with 99% identity.

Insert lengths(kb): 2.5

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: testis enhanced gene transcript(TEGT) TEGT

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII/NotI--3.3, 1.75, 0.8.

Corresponds to nt 58-2566 of GenBank X75861, and contains most of the coding sequence (nt 41-754).

Verified to contain EST06590 by single-pass sequencing.

Related product(s): oligonucleotides, ATCC 73233.