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| 产品名称： | pOSEX3 |
|---|---|
| 商品货号： | TS146322 |
| Designations： | pOSEX3 |
| Depositors： | E Bremer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4320001602172850 Vector: pOSEX3 (plasmid) Promoters: Promoter for expression proU Construction: pOSEX2 Marker(s):ampR Construct size (kb): 4.432000160217285 Features: marker(s): ampR promoter for expression: proU replicon: pMB1 replicon: rop (copy number control) MCS: BamHI...HindIII transcription terminator: rrnB T1 transcription terminator: rrnB T2 coding sequence: proV 5 sequence |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4. Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride. Vector does not provide a ribosome binding site or initiation codon. Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter. This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor. Vector constructed from pOSEX2 (ATCC 87211) by deletion of a portion of the pBR322 backbone. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862 Erhard Bremer, personal communication |
| Shipped： | freeze-dried |