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| 产品名称： | pNF3 |
|---|---|
| 商品货号： | TS146323 |
| Designations： | pNF3 |
| Depositors： | EC Friedberg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: pNF3 (plasmid) Promoters: Promoter nifH Construction: pNF2 Marker(s):G418R,URA3,ampR,kanR Construct size (kb): 7.0 Features: marker(s): ampR, kanR, G418R, URA3 marker(s): ampR, kanR, spcR, strR promoter: nifH replicon: pMB1 terminator: none |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | This plasmid is identical to pNF2 (ATCC 37187) except for the deletion of the 2.2 kb EcoRI fragment containing the yeast 2 micron origin of replication. SalI sites flank the BamHI cloning site for ready removal of the insert. A integration shuttle vector for subcloning and recovery of DNA from Saccharomyces cerevisiae. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Naumovski L, Friedberg EC. Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments. Gene 22: 203-209, 1983. PubMed: 6307821 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Shipped： | frozen |