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| 产品名称： | pbPKC-gamma19 |
|---|---|
| 商品货号： | TS146359 |
| Designations： | pbPKC-gamma19 |
| GenBank Number： | M13976 |
| Species： | Bos taurus, cow |
| Depositors： | A Ullrich |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.911999940872192 Tissue: brain Gene product: protein kinase C, gamma PRKCG |
| Insert Size (kb)： | 1.912 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following restriction sites (with positions according to the published sequence): PstI--678, 1581. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; PstI--3.7, 0.9; HindIII--4.6. The probe detects bovine cerebellar mRNA of 3.1 and 2.4 kb and rat brain mRNA of 3.1 kb. Used to map the human gene. It detects human genomic HindIII fragments of 5.5, 4.6, and 4.3 kb and EcoRI fragments of 7.2, 4.4, and 3.4 kb. A 170 bp HincII/EcoRI fragment of the insert detects a major genomic bovine PvuII fragment of 10.5 kb and weaker signals at 5.7 and 7.6 kb. In humans this fragment detects a genomic 7.2 kb PvuII fragment. |
| References： | Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548 |