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| 产品名称： | pZJMalpha |
|---|---|
| 商品货号： | TS146423 |
| Designations： | pZJMalpha |
| Depositors： | J Morris |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.3710000000000000 Vector: pZJMalpha (plasmid) Type: expression Construction: A 656bp fragment of tubulin is cloned into XhoI/HindIII site. Construct size (kb): 6.4 Features: marker(s): ampR, BleoR operator: Tet promoter: T7 replicon: E. coli Ori MCS: XhoI, XbaI, NdeI, HpaI, HindIII, SfiI terminator: T7 transcription terminator: Actin3 UTR, Aldolase 3 UTR |
| Applications： | vector for studying DNA-protein interactions tubulin, alpha |
| Comments： | PCR-amplified gene fragments can be ligated in a single step between two opposing T7 promoters. This vector can integrate into a nontranscribed region of the Trypanosoma brucei genome and establish a stable cell line. Tetracycline induction leads to the production of a dsRNA with concomitant degradation of the target mRNA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Wang Zefeng, et al. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275: 40174-40179, 2000. PubMed: 11013266 |
| Shipped： | frozen |