微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRSVZ |
|---|---|
| 商品货号： | TS146441 |
| Designations： | pRSVZ |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.8000001907348630 Vector: pRSVZ (plasmid) Promoters: Promoter RSV LTR Construction: pRSVcat, pC4AUGbeta-gal Marker(s):ampR Construct size (kb): 7.800000190734863 Features: insert detection: lacZ marker(s): ampR promoter: RSV LTR replicon: pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 2.6; XbaI--7.8. Produces beta-gal under control of RSV LTR, which functions in both Escherichia coli & mammalian tissue culture cells. Co-transfect with other plasmids and determine relative transfection efficiency by normalizing to beta-gal activity. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, et al. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of bacterial gene. Somatic Cell Mol. Genet. 13: 253-265, 1987. PubMed: 2440117 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |