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微信小章| 产品名称： | Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human |
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| 商品货号： | TS146450 |
| Organism： | Homo sapiens, human |
| Tissue： | Blood |
| Cell Type： | Mononuclear |
| Morphology： | spherical; variable after culturing |
| Growth Properties： | suspension; variable after culturing |
| Biosafety Level： | 1 xa0These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.xa0 ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided inxa0Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC)xa0(Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication:xa0Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online atxa0http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.xa0 Human Material Precautionxa0 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | lot specific |
| Gender： | lot specific |
| Ethnicity： | lot specific |
| Applications： | Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay. |
| Product Format： | frozen 1 mL |
| Storage Conditions： | -130°C |
| Images： |  |
| Comments： | Peripheral blood mononuclear cells (PBMC) have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.xa0
Peripheral blood mononuclear cells are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes. |
| Complete Growth Medium： | Peripheral blood mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.
Note: Application specific medium, cytokines and other factors |
| Subculturing： | N/A |
| Volume： | 1 mL |
| Cells per Vial： | One vial contains a minimum of 2.5 x 107 viable cells. |
| Viral Testing： | Hepatitis B:xa0Negative
Hepatitis C:xa0Negative
HIV(I/II):xa0Negative
HTLV(I/II):xa0Negative
WNV:xa0Negative
Trypanasome:xa0Negative |
| Viability： | ≥ 70% when thawed from cryopreservation |
| Population Doubling Capacity： | N/A |
| C of A： |  Certificate of Analysis |