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| 产品名称： | p16R1 |
|---|---|
| 商品货号： | TS146561 |
| Designations： | p16R1 |
| Depositors： | DB Young |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0999999046325680 Vector: p16R1 (plasmid) Construction: pAL5000, pIJ963 Marker(s):hygR Construct size (kb): 5.099999904632568 Features: marker(s): hygR replicon: pAL5000 restriction site: KpnI |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.4; KpnI--5.1; BglII--4.0, 1.2. Mycobacteria containing this vector should be grown on 7H9/7H11 media plus hygromycin (50 mcg/ml). Shuttle vector allowing transformation of a broader range of Mycobacterial species and at a higher efficiency than the kanamycin resistance confering shuttle vectors pBAK14, pYUB12 and pMV251. Constructed by insertion of the pAL5000 origin of replication at the BamHI site of pIJ963, followed by subsequence loss of a 1.9 kb fragment including the ampR region. |
| Media： | ATCC® Medium 1952: LB medium (ATCC medium 1065) with 200 mcg/ml hygromycin B |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Garbe TR, et al. Transformation of mycobacterial species using hygromycin resistance as selectable marker. Microbiology 140: 133-138, 1994. PubMed: 8162182 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried plasmid in E. coli |