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微信小章| 产品名称： | B35 |
|---|---|
| 商品货号： | TS146607 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | central nervous system (CNS) |
| Cell Type： | neuronal neuroblast; nitrosoethylurea (NEU) induce |
| Product Format： | frozen |
| Morphology： | neuronal |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 4 to 10 months |
| Strain： | BD1X |
| Applications： | B35 cells can be stimulated to differentiate in the presence of dibutyryl cyclic AMP (cAMP) or by serum deprivation. The cells also may be used to study the metabolism and physiology of nervous tissue and the pathology of nervous disorders. They are easily transfected with plasmid DNA. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Rats were inoculated with N-nitrosoethylurea (NEU) 15 days after conception. Tumors found in the central nervous system (CNS) 4 to 10 months after birth were excised, minced, adapted to culture and cloned. Ref xad |
| Comments： | The cells retain glutamic acid decarboxylase (GAD) and choline acetyltransferase activities; express gamma aminobutyric acid (GABA). The cells are negative for S100 (S-100) protein. Ref A culture submitted to the ATCC in October 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: Subculture before confluency.
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | P Maness |
| References： | Schubert D, et al. Clonal cell lines from the rat central nervous system. Nature 249: 224-227, 1974. PubMed: 4151463 Vinores SA, et al. Immunoradiometric and immunohistochemical demonstration of neuron-specific enolase in experimental rat gliomas. Cancer Res. 44: 2595-2599, 1984. PubMed: 6722796 Otey CA, et al. B35 neuroblastoma cells: an easily transfected, cultured cell model of central nervous system neurons. Methods Cell Biol. : 287-304, 2003. PubMed: 12884695 |
Schubert D, et al. Clonal cell lines from the rat central nervous system. Nature 249: 224-227, 1974. PubMed: 4151463