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| 产品名称： | HRRM1 STSG-10564 |
|---|---|
| 商品货号： | TS146665 |
| Designations： | HRRM1 STSG-10564 |
| GenBank Number： | X59617 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.329999923706055 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCRII Intact vector size: 3.900 Type of vector: phagemid Vector end: TA site Vector end: TA site Cloning sites: TA cloning between EcoRI sites Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR, kanR promoter: lac, SP6, T7 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: RRM1 Genomic copy number: unique Gene name: ribonucleotide reductase M1 polypeptide Contains complete coding sequence?: Y Chromosome: 11; Localization: 11 p15.5-p15.4 Type of DNA: cDNA Insert 5 end: Modification: SacI site Insert 3 end: Modification: PstI site Insert size (kb): 2.379 Cross references: DNA Seq. Acc.: X59617 Nucleotides ~1~2379 of the insert correspond to nucleotides 182-2560 of X59617. Insert lengths(kb): 2.378999948501587 Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) RRM1 Alleles: A2, A2, A1 |
| Insert Size (kb)： | 2.379 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.7; SacI--4.0, 2.3; PstI--2.7, 2.4, 1.2; HindIII--3.7, 2.3; PstI/SacI--2.7, 2.4, 1.2. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.07; EcoRI--0.39, 0.93, 1.69; BamHI--0.53, 2.37. Constructed by PCR using primers generating a SacI site just upstream of the initation codon (5 GCTCGAGCTCATG 3) and a PstI site just after the termination codon (5 TGAG CTGCAGCTGC 3). |
| References： | Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826 Yun Yen, personal communication |