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| 产品名称： | YRp8phocat |
|---|---|
| 商品货号： | TS146671 |
| Designations： | YRp8phocat |
| Depositors： | H Feldmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.9899997711181640 Vector: YRp8phocat (plasmid) Promoters: Promoter PHO5 Construction: YRp7-32phocat (ATCC 37625), URA3 Marker(s):TRP1,URA3,ampR Construct size (kb): 7.989999771118164 Features: marker(s): ampR, TRP1, URA3 promoter: PHO5 replicon: pMB1, ARS1 |
| Applications： | YR-type (replicating) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--6.1, 1.95; BglII--8.2; EcoRI--5.8, 1.45, 0.60. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 6015 bp BamHI/HindIII fragment of YRp8 containing URA3 was ligated to a 1975 bp BamHI/HindIII fragment of YRp7-32phocat (ATCC 37625). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257 |
| Shipped： | freeze-dried |