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微信小章| 产品名称： | pcD-hIL-4 |
|---|---|
| 商品货号： | TS146717 |
| Designations： | pcD-hIL-4 |
| GenBank Number： | M13982 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.839999914169312 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7200000286102295 Tissue: helper T-cell 2F1 cell line, Con A-stimulated Gene product: interleukin 4 IL4 |
| Insert Size (kb)： | 0.720 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.8; HindIII--3.8; PstI--2.3, 1.6; ClaI--3.8; XhoI--2.8, 0.9. Exact restriction sizes are as follows: BamHI--2.98 kb, 0.86 kb; HindIII--3.84 kb; PstI--2.27 kb, 1.57 kb; ClaI--3.84 kb; XhoI--2.92 kb, 0.92 kb. The PstI site given in the paper at the 5 end does not exist. The plasmid contains the PstI site in bla and a PstI site in the insert. Interleukin 4 and interleukin 5 are encoded in the same 345 kb NotI fragment. Contains about 100 bp of poly(A). The insert contains the complete coding sequence and includes NheI, EcoRV, PstI, PvuII, BglI, and EcoRI sites. |
| References： | Chandrasekharappa SC, et al. A long-range restriciton map of the interleukin-4 and interleukin-5 linkage group on chromosome 5. Genomics 6: 94-99, 1990. PubMed: 2303264 Yokota T, et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 5894-5898, 1986. PubMed: 3016727 Takashi Yokota, personal communication |