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微信小章| 产品名称： | GMCSF pCSF-1 |
|---|---|
| 商品货号： | TS146746 |
| Designations： | GMCSF pCSF-1 |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc., GG Wong, Genetics Institute, Inc. |
| Applications： | in another host, produces protein colony stimulating factor 2 (granulocyte-macrophage) |
| Vector： | Construct size (kb): 9.75 Name of vector: p91023(B) Intact vector size: 8.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: SV40 marker(s): tetR promoter: Ad2 major late replicon: pMB1, SV40 terminator: SV40 |
| Insert： | DNA: cDNA Genome: human Gene symbol: CSF2 Genomic copy number: unique Gene name: colony stimulating factor 2 (granulocyte-macrophage) Contains complete coding sequence?: U Chromosome: 5; Localization: 5 q23-q31 Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066) Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 0.8 Insert lengths(kb): 0.80 Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066) Gene product: colony stimulating factor 2 (granulocyte-macrophage) CSF2 Target Gene: colony stimulating factor 2 (granulocyte-macrophage) |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.0, 0.81; HindIII--4.8, 1.6, 1.3, 0.6. This clone may be most reliably recovered by streaking on tetracycline-containing plates, picking single colonies, and doing a miniprep. Contains 8 bp 5 untranslated; ORF of 432 nucleotides encoding 144 amino acids (complete coding sequence), and 3 untranslated sequences. The vector allows expression in COS cells. The insert contains PstI, BglI, NcoI and AhaIII sites. |
| References： | Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987 Rajagopalan LE, Malter JS. Turnover and translation of in vitro synthesized messenger RNAs in transfected, normal cells. J. Biol. Chem. 271: 19871-19876, 1996. PubMed: 8702698 Wong GG, et al. Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228: 810-815, 1985. PubMed: 3923623 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |