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| 产品名称： | pGX2907 GX3305, GX3320 |
|---|---|
| 商品货号： | TS146749 |
| Designations： | pGX2907 GX3305, GX3320 |
| Species： | Staphylococcus aureus Rosenbach |
| Depositors： | Genex Corp. |
| Vector： | Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGX284 Intact vector size: 2.150 Type of vector: plasmid Vector end: EcoRV Vector end: EcoRV Cloning sites: EcoRV Polylinker sites: Construction: pBR322, pC194, bacteriophage lambda, Bacillus subtilis Host range: Bacillus subtilis; Escherichia coli Features (with orientation and position when available): marker(s): ampR, cmlR replicon: pMB1 terminator: lambda Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Staphylococcus aureus Gene symbol: pra Gene name: protein A Contains complete coding sequence?: U Genome: Staphylococcus aureus Gene symbol: pra Gene name: protein A Contains complete coding sequence?: U Strain: Cowan I Type of DNA: genomic Insert end: EcoRV Insert end: EcoRV Insert size (kb): 2.15 Cross references: Insert lengths(kb): 2.150000095367432 Gene product: protein A pra |
| Insert Size (kb)： | 2.150 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--8.8, 2.15; PstI--5.6, 3.1, 2.3; HindIII--9.4, 1.6; PvuI--8.8, 2.15; BamHI--uncut. This construct is available in two forms: in Escherichia coli as the plasmid (TS146749) and integrated into the Bacillus chromosomal DNA (ATCC 39345). The depositor suggests streaking for single colonies on L broth + 1.5% agar + 100 ug/ml ampicillin + 5 ug/ml chloramphenicol and using a single colony. The vector contains the replicon and beta-lactamase gene from pBR322, a galactokinase gene, a CAT gene from pC194, and a transcription terminator from bacteriophage lambda. It also contains chromosomal sequences from Bacillus subtilis for integration. |
| References： | Fahnestock SR. Production of protein A. US Patent 4,617,266 dated Oct 14 1986 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |