微信小陈
微信小章| 产品名称： | Dictyostelium discoideum Raper |
|---|---|
| 商品货号： | TS146813 |
| Strain Designations： | Ax-3 |
| Application： | produces calmodulin transformation host hydrolysis of a phosphonate ester |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Comments： | Actin binding to plasma membrane
Thymidine kinase activity
Part of the Eumycetozoan Project
This strain may grow in axenic medium or with Klebsiella aerogens, but ATCC maintains this strain with a bacterial food source. |
| Medium： | ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X) ATCC® Medium 671: Dictyostelium medium |
| Growth Conditions： | Temperature: 20°C to 25°C Atmosphere: Typical aerobic |
| Sequenced Data： | No DNA sequencing was performed in house on this product.
|
| Name of Depositor： | WF Loomis |
| Special Collection： | Part of Eumycetozoan Project |
| Chain of Custody： | ATCC |
| Cross References： | Nucleotide (GenBank) : D12883 gene coding for gp138 Nucleotide (GenBank) : D12884 gene coding for gp138 |
| References： | Loomis WF Jr.. Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Exp. Cell Res. 64: 484-486, 1971. PubMed: 5542656 Yarger J, et al. Observations on the growth of Dictyostelium discoideum in axenic medium: evidence for an extracellular growth inhibitor synthesized by stationary phase cells. J. Cell Sci. 14: 681-690, 1974. PubMed: 4857469 Fey P, et al. Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum. Gene 165: 127-130, 1995. PubMed: 7489901 Kessin RH, et al. Linkage analysis in Dictyostelium discoideum using temperature- sensitive growth mutants selected with bromodeoxyuridine. J. Bacteriol. 119: 776-783, 1974. PubMed: 4859399 Michrina CA, Deering RA. Thymidine kinase activity in Dictyostelium discoideum. J. Gen. Microbiol. 119: 263-266, 1980. PubMed: 7411120 Jacobson BS. Actin binding to the cytoplasmic surface of the plasma membrane isolated from Dictyostelium discoideum. Biochem. Biophys. Res. Commun. 97: 1493-1498, 1980. PubMed: 6894237 Fang H, et al. Molecular cloning and characterization of two genes encoding gp138, a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum. Dev. Biol. 156: 201-208, 1993. PubMed: 7680629 Rossomando EF, Jahngen JH. Hydrolysis of a phosphonate ester catalyzed by an enzyme from Dictyostelium discoideum. Arch. Biochem. Biophys. 197: 364-366, 1979. PubMed: 232405 Clarke M, et al. Isolation and properties of calmodulin from Dictyostelium discoideum. J. Bacteriol. 141: 397-400, 1980. PubMed: 6243626 Bazari WL, Clarke M. Characterization of a novel calmodulin from Dictyostelium discoideum. J. Biol. Chem. 256: 3598-3603, 1981. PubMed: 6259174 Loomis WF. Dictyostelium discoideum. A developmental system. Academic Press, New York, 1975. |