微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pcDV1 |
|---|---|
| 商品货号： | TS146818 |
| Designations： | pcDV1 |
| Depositors： | H Okayama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0000000000000000 Vector: pcDV1 (plasmid) Promoters: Promoter none Construction: pBR322, SV40 Marker(s):ampR Construct size (kb): 3.0 Features: marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 late enhancer: none |
| Applications： | expression vector vector for constructing cDNA libraries |
| Comments： | pcDV1 (TS146818) and pL1 (ATCC 37369) should be used together to construct a cDNA library. The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end and the polyadenylation sequence from the late region of the virus. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662 |
| Related Products： | component of:ATCC 77361 |
| Shipping Information： | Distributed: freeze-dried |