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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS146835 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | PMC107 |
| Application： | Transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | mcrA delta(mcrBC-hsdRMS-mrr)102 recB21 recC22 recJ154 sbcB15 sbcC201 |
| Type Strain： | no |
| Comments： | Allows the recovery of bacteriophage with recombinant DNA containing secondary structure, but supports poorer phage growth than PMC103 and PMC128 (ATCC® 47060™, 47064™). This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product (personal communication). Constructed from JC12186 by P1 transduction of delta(mcrBC-hsdRSM-mrr) from Escherichia coli ER1648. Methylation tolerant, recombination deficient host that improves the stability of cosmid clones |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | JP Doherty |
| References： | Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479 Judith P Doherty, personal communication |