Construct size (kb): 5.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCGR*
Gene name: secretogranin II
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: NotI
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M25756; EST: EST06224
Nucleotides 1-390 of the insert correspond to
nucleotides 721-1110 of M25756 with 98% identity.
DNA Seq. Acc.: T08333; EST: EST06224; Primer: M13 Reverse;
THC id: THC85513

Insert lengths(kb): 1.700000047683716

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: secretogranin II SCGR*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.7, EcoRI--5.0.

Based on the alignment with GenBank accession M25756 (2336 nt, CDS 63-1916), this clone appears to contain part of the coding sequence.

The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII.