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| 产品名称： | pAA3H |
|---|---|
| 商品货号： | TS146926 |
| Designations： | pAA3H |
| Depositors： | A Ahmed |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.5000000000000000 Vector: pAA3H (cosmid) Promoters: Promoter none Construction: pBR322, lambdagal+(EcoRI/HindIII, galETK-cos R/L) Marker(s):ampR,galS Construct size (kb): 11.5 Features: insert detection: galETK marker(s): ampR, galS promoter: none replicon: pMB1 terminator: none enhancer: none |
| Applications： | vector for constructing genomic libraries vector permitting positive selection for inserts |
| Comments： | The galETK genes are carried on a HindIII/HpaI fragment of approximately 3.0 kb. The galE gene is truncated at the HindIII site. Expression of the gal sequences are regulated by a promoter located between the BamHI and HindIII sites in the tet gene. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Vector for positive galactose-resistance selection of DNA cloned into the HindIII site. Derived from pAA3. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Ahmed A. Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli. Gene 28: 37-43, 1984. PubMed: 6329914 |
| Shipped： | freeze-dried |