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| 产品名称： | pLG117R |
|---|---|
| 商品货号： | TS146965 |
| Designations： | pLG117R |
| Species： | Homo sapiens, human |
| Depositors： | Harvard Univ. |
| Applications： | produces protein interferon, beta 1, fibroblast |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR Cross references: DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNB1 Other gene symbols: IFNB Genomic copy number: unique Gene name: interferon, beta 1, fibroblast Contains complete coding sequence?: U Chromosome: 9; Localization: 9 p22 Tissue: fibroblast Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.6 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNB1 Other gene symbols: IFNB Genomic copy number: unique Gene name: interferon, beta 1, fibroblast Contains complete coding sequence?: U Chromosome: 9; Localization: 9 p22 Tissue: fibroblast Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.6 Cross references: Insert lengths(kb): 0.6000000238418579 Tissue: fibroblast Gene product: interferon, beta 1, fibroblast IFNB Target Gene: interferon, beta 1, fibroblast |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains an internal PstI site. Restriction digests of the clone give the following sizes (kb): BamHI/BglII--2.2, 0.6; PstI--1.8, 0.96; BamHI--2.8. Encodes a 20 kDa protein product in Escherichia coli, corresponding to the expected molecular weight of unglycosylated interferon beta. Demonstrates anti-viral activity (inhibition of cytopathic effect using VSV and human fibroblasts). Constructed by inserting a lacZ promoter (derivative of lac95 portable promoter) upstream of Bal31-digested pre-interferon beta cDNA. |
| References： | Taniguchi T, et al. Expression of the human fibroblast interferon gene in Escherichia coli. Proc. Natl. Acad. Sci. USA 77: 5230-5233, 1980. PubMed: 6159642 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |