宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pRIL6RC.6

货号 TS146981
中文名称 null
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产品名称: pRIL6RC.6
商品货号: TS146981
Designations: pRIL6RC.6
GenBank Number:

J05668

Species: Rattus norvegicus, rat
Depositors: GH Fey
Vector:
Construct size (kb): 7.300000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 4.590000152587891
Tissue: liver, acute inflammation
Gene product: interleukin 6 receptor IL6R
Insert Size (kb): 4.590
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
The insert includes the following restriction sites (in nucleotide positions from the 5 end of the published sequence): BamHI--748, 1083, 1177; PstI--1586, 3524, 3999, 4060; XbaI--2359.
Restriction digests of the clone give the following sizes (kb): BamHI--6.4, 0.8; EcoRI--4.6, 2.7; HindIII--3.2, 2.2, 1.3, 0.7; PstI--3.1, 1.9, 1.5, 0.5; XbaI--5.2, 2.4.
The insert extends from nucleotide 71 to 4661 of the published sequence, and encodes the complete reading frame (109 - 1495), plus 5 and 3 untranslated regions.
This detects a mRNA of about 5.1 kb in normal and acute-phase rat liver.
References:

Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054