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| 产品名称： | pBS-hCD40L-6A9 |
|---|---|
| 商品货号： | TS147009 |
| Designations： | pBS-hCD40L-6A9 |
| GenBank Number： | L07414 |
| Species： | Homo sapiens, human |
| Depositors： | J Gauchat |
| Vector： | Construct size (kb): 4.059999942779541 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, T7, T3 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD40LG Other gene symbols: CD40L Gene name: CD40 antigen ligand Contains complete coding sequence?: Y Chromosome: X; Localization: X q26 Tissue: CD4-positive T-cells Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.1 Cross references: DNA Seq. Acc.: L07414 Insert lengths(kb): 1.100000023841858 Tissue: CD4-positive T-cells Gene product: CD40 antigen ligand CD40L |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 0.2; HindIII--3.3, 0.7; NcoI--4.0; PvuII--2.6, 1.0, 0.6; EcoRI--3.0, 1.2. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.93; HindIII--0.58; NcoI--0.78; PvuII--0.52. Contains the complete coding sequence (nt 40-825 of the sequence record). |
| References： | Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552 Jean-Francois Gauchat, personal communication |