| 产品名称: | pE25.a pBS300 |
|---|---|
| 商品货号: | TS147022 |
| Designations: | pE25.a pBS300 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 3.09500002861023 |
| Insert: | DNA: genomic Insert lengths(kb): 0.3490000069141388 Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line Gene product: DNA Segment, repetitive D1Z7 |
| Insert Size (kb): | 0.349 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments: | The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227). Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6. The first half of the dimer is 85% identical to the sequence of the 1 monomer; the second half is 80% identical to the 2 monomer. The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof. |
| References: | Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415 Immo E Scheffler, personal communication |