宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pE25.a [pBS300]

货号 TS147022
中文名称 null
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产品简介
购买须知
产品名称: pE25.a pBS300
商品货号: TS147022
Designations: pE25.a pBS300
Species: Homo sapiens, human
Vector:
Construct size (kb): 3.09500002861023
Insert:
DNA: genomic
Insert lengths(kb): 0.3490000069141388
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive D1Z7
Insert Size (kb): 0.349
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6.
The first half of the dimer is 85% identical to the sequence of the 1 monomer; the second half is 80% identical to the 2 monomer.
The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof.
References:

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Immo E Scheffler, personal communication