微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pcD-hGM/Eo-CSF pcD-hGM |
|---|---|
| 商品货号： | TS147073 |
| Designations： | pcD-hGM/Eo-CSF pcD-hGM |
| GenBank Number： | M11220 |
| Species： | Homo sapiens, human |
| Depositors： | T Yokota |
| Vector： | Construct size (kb): 3.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8899999856948853 Gene product: colony stimulating factor 2 (granulocyte-macrophage) CSF2 |
| Insert Size (kb)： | 0.890 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.05; HindIII--3.9; PstI--2.6, 1.3; ClaI--3.9; XhoI--2.8, 1.1. Exact restriction sizes are as follows: BamHI--2.98 kb, 1.03 kb; HindIII--4.01 kb; PstI--2.59 kb, 1.295 kb; ClaI--4.01 kb; XhoI--2.92 kb; 1.09 kb. Contains the complete coding sequence and a poly(A) tract of 0.1 kb. The insert contains PstI, BglI, and ApaI sites in the coding region and NcoI and AhaIII sites in the non-coding region. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Lee F, et al. Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells. Proc. Natl. Acad. Sci. USA 82: 4360-4364, 1985. PubMed: 3925454 Barbara Weiffenbach, personal communication Takashi Yokota, personal communication |