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| 产品名称： | p8.1.1 |
|---|---|
| 商品货号： | TS147106 |
| Designations： | p8.1.1 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.735000014305115 Tissue: placenta (34 weeks) Gene product: keratin 8 KRT8 |
| Insert Size (kb)： | 1.735 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; HindIII--3.3, 1.1; BamHI--4.4; PstI--3.1, 0.7, 0.4; EcoRI/HindIII--2.7, 1.1, 0.6. There is an error in the reported sequence at nt 642 which is a T not an A. The encoded amino acid (Ala) remains the same. The change explains the HindIII restriction site yielding a 1.1 kb fragment. The clone contains the complete coding sequence including 33 bp 5 untranslated and 250 bp 3 untranslated regions. The clone detects a single mRNA species of approximately 1.9 kb in fetal intestine, placenta, pancreas, lung, liver, heart, and kidney with little in adrenal gland or muscle. |
| References： | Yamamoto R, et al. Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP. Mol. Endocrinol. 4: 370-374, 1990. PubMed: 1692965 Jerome F Strauss, personal communication |