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| 产品名称： | 2P1 |
|---|---|
| 商品货号： | TS147231 |
| Designations： | 2P1 |
| Species： | Homo sapiens, human |
| Depositors： | F Kao |
| Vector： | Chromosome: 2p23-p25 DNA: genomic Digest: Complete Insert size range: 50-600 bp |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Approximately 40% of the clones contain repetitive sequences. Of 48 single copy clones tested, 26 (54%) hybridized to chromosome 2. Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19. |
| References： | Yu J, et al. A region-specific microdissection library for human chromosome 2p23-p25 and the analysis of an interstitial deletion of p23.3-p25.1. Hum. Genet. 93: 557-562, 1994. PubMed: 8168834 |