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微信小章| 产品名称： | phpep14-21 |
|---|---|
| 商品货号： | TS147233 |
| Designations： | phpep14-21 |
| Species： | Homo sapiens, human |
| Depositors： | R Taggart |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7049999833106995 Tissue: gastric mucosa Gene product: pepsinogen PGA* |
| Insert Size (kb)： | 0.705 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.75; HindIII--5.2; EcoRI--5.2; AvaI--5.2; BamHI--5.2. Detects genomic restriction fragments of the following sizes (kb): owl monkey BamHI--13.0, 3.6. Insert corresponds to bases 332 - 1017 of predicted 1374 mRNA sequence and amino acids 80 - 307. Includes a portion of exon 3 through exon 8. Exon 3-5 and 6-8 specific probes can be generated by HaeII digestion. The 17.2 kb EcoRI genomic fragment is detected by the exon 3 - 5 probe but not the 6 - 8 probe. The 17.2 kb EcoRI fragment is detected in haplotypes A (PGA3, PGA4, PGA5 genes) and B (PGA3, PGA4 genes). The 3.9 kb EcoRI genomic fragment is detected by the exon 6 - 8 probe but not the exon 3 - 5 probe. This fragment is a doublet resulting from two distinct PGA genes. The 13.5 kb EcoRI genomic fragment is detected by the exon 3 - 5 probe but not the exon 6 - 8 probe. This EcoRI fragment is detected in haplotype A which contains the PGA3, PGA4, and PGA5 genes. The 22.0 kb and the 17.8 kb genomic EcoRI fragments are detected by both the exon 3 - 5 and 6 - 8 probes, suggesting the size heterogeneity results from variation at the 5 region of two distinct genes. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Ma NS, et al. Homologs of eleven loci on human chromosome 11 assigned to two owl monkey chromosomes. Cytogenet. Cell Genet. 56: 206-211, 1991. PubMed: 2055119 Taggart RT, et al. Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism. Proc. Natl. Acad. Sci. USA 82: 6240-6244, 1985. PubMed: 3862130 Taggart RT, et al. Relationships between the human pepsinogen DNA and protein polymorphisms. Am. J. Hum. Genet. 38: 848-854, 1986. PubMed: 3014868 Barbara Weiffenbach, personal communication |