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| 产品名称： | pIIB1 |
|---|---|
| 商品货号： | TS147319 |
| Designations： | pIIB1 |
| GenBank Number： | X59268 |
| Species： | Homo sapiens, human |
| Depositors： | D Reinberg |
| Vector： | Construct size (kb): 4.619999885559082 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.620000004768372 Tissue: HeLa cell line Gene product: transcription factor IIB(RNA polymerase II transcription factor IIB) TCF2B* |
| Insert Size (kb)： | 1.620 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.6; AccI--3.3, 0.86, 0.3; XhoI--3.4, 0.76, 0.46; BamHI--4.6; PvuII--2.4, 1.5, 0.64. The insert contains the following restriction sites (approximate kb from the 5 cloning site): EcoRI--0.03, 0.04, 0.12; AccI--0.4, 0.64, 0.9; BstXI--0.41; XhoI--0.6, 1.28. Detects human genomic restriction fragments of the following sizes (approximate kb): BamHI--23; EcoRI--10; HindIII--15; Contains the complete coding sequence, with 5 and 3 untranslated regions. and an mRNA in HeLa cells of 1.7 kb. There are about 40 bp between the 5 reported sequence and the vector cloning site (adjacent to the T3 promoter). This region contains 3 EcoRI sites. |
| References： | Ha I, et al. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature 352: 689-695, 1991. PubMed: 1876184 Danny Reinberg, personal communication |